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<title>UTas ePrints - Steroids and Reproductive Biology in the Blotched Blue-tongued Lizard, Tiliqua nigrolutea</title>
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<meta content="Edwards, Ashley" name="eprints.creators_name" />
<meta content="Ashley.Edwards@utas.edu.au" name="eprints.creators_id" />
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<meta content="2007-02-02" name="eprints.datestamp" />
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<meta content="Steroids and Reproductive Biology in the Blotched Blue-tongued Lizard, Tiliqua nigrolutea
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<meta content="270604" name="eprints.subjects" />
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<meta content="This thesis documents the annual profiles of the primary reproductive steroids testosterone (T), 17beta-oestradiol (E2) and progesterone (P4), in the reproductive cycles of male and female blue-tongued lizards, Tiliqua nigrolutea. Data collected from a large captive population over three consecutive reproductive seasons are included. Reproductive cycles are discussed in the context of other viviparous squamate reptiles, while a broader comparative approach is used to consider patterns of steroid biosynthesis and peripheral metabolism.
The annual patterns of circulating concentrations of T, E2 and P4 have been characterised for both sexes. In males, peak plasma T (10.9 +/- 3.00 ng ml-1) and E2 (778.0 +/- 120.00 pg ml-1) concentrations occur coincident with late spermatogenesis and observations of mating, respectively. Plasma P4 concentrations remain basal (< 1.2 ng ml-1) throughout the annual reproductive cycle. In females, increasing plasma E2 concentrations (275.2 +/- 33.87 pg ml-1 - 715.1 +/- 106.68 pg ml-1) are associated with vitellogenesis and plasma T peaks (6.3 +/- 0.63 ng ml-1) in the mating and peri-ovulatory period. In pregnant females, plasma P4 concentrations are elevated for the first two thirds of gestation, peaking in the second trimester at 12.7 +/- 1.27 ng ml-1 and falling rapidly prior to parturition. Concurrently, plasma P4 concentrations in non-reproductively active adult females remain basal (1 - 2 ng ml-1) throughout the year.
There is good circumstantial evidence for a multiennial reproductive cycle in females. Parturition occurs late in the active season, presumably leaving little time for females to store sufficient fat reserves to become vitellogenic in the following spring: reproductive opportunities are effectively missed in at least one year following a reproductive effort. Observed reproductive behaviours, including agonistic male - male interactions, mating, and parturition, are documented.
An investigation of gonadal steroid biosynthetic pathways in this viviparous squamate is presented. This compares variation in the relative contributions of the delta-4 and delta-5 steroidogenic pathways according to sex and reproductive condition. The delta-4 pathway predominates in both sexes, aligning this species phylogenetically with other reptiles. However, there are clear differences between sexes and with changing reproductive condition in the patterns of production of pathway intermediates and end-products. Additionally, detection of a possibly novel polar steroid as a major end-product of steroid biosynthesis in both sexes is reported.
Peripheral (extragonadal) metabolism of T and E2 in a number of reproductively relevant steroid target tissues is compared at times of year chosen to represent three clearly distinctive reproductive conditions in each sex. There are differences both between sexes, between tissue types and with changing reproductive condition in the relative proportions of steroid conjugates and non-conjugated derivatives produced. Biosynthetic pathway activity and peripheral steroid metabolism both appear to be plastic in response to changing reproductive condition in Tiliqua nigrolutea.
With a comprehensive database of information about the reproductive endocrinology and physiology of Tiliqua nigrolutea, this species is now available as a model to further examine selected aspects of the steroid hormone control of reproductive physiology and behaviour in a cool temperate, viviparous reptile.
" name="eprints.abstract" />
<meta content="2000" name="eprints.date" />
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<meta content="217" name="eprints.pages" />
<meta content="University of Tasmania" name="eprints.institution" />
<meta content="School of Zoology" name="eprints.department" />
<meta content="phd" name="eprints.thesis_type" />
<meta content="Abell A.J. (1998). The effect of exogenous testosterone on growth and secondary sexual character development in juveniles of Sceloporus virgatus. Herpetologica 54 533-543.
Abts M.L. (1988). Reproduction in the saxicolous desert lizard, Sauromalus obesus: the female reproductive cycle. Copeia 1988 382-393.
Adkins-Regan E. (1981). Hormone specificity, androgen metabolism and social behaviour. Amer. Zool. 21 257-271.
Alberts A.C. (1990). Chemical properties of femoral gland secretions in the desert iguana, Dipsosaurus dorsalis. J. Chem. Ecol. 16 13-25.
Aldridge R.D. (1979). Female reproductive cycles of the snakes Arizona elegans and Crotalus viridis. Herpetologica 35 256-261.
Aldridge R.D. (1982). The ovarian cycle of the watersnake Nerodia sipedon, and effects of hypophysectomy and gonadotropin administration. Herpetologica 38 72-79.
Aldridge R.D. (1992). Oviductal anatomy and seasonal sperm storage in the south-eastern crowned snake (Tantilla coronata). Copeia 1992 1103-1106.
Aldridge R.D. and Brown W.S. (1995). Male reproductive cycle, age at maturity, and cost of reproduction in the timber rattlesnake (Crotalus horridus). J. Herpetol. 29 399-407.
Ananthalakshmi M.N., Devaraj Sarkar H.B. and Shivabasavaiah. (1991). Experimental demonstration of androgen regulation of hemipenis in the lizard, Calotes versicolor. Zool. Sci. 8 561-566.
Ando S., Ciarcia G., Panno M.L., Imbrogno E., Tarantino G., Buffone M., Beraldi E., Angelini F. and Botte V. (1992). Sex steroids in the plasma and testis during the reproductive cycle of the lizard Podarcis s. sicula Raf. Gen. Comp. Endocrinol. 85 1-7.
ANZCCART (1993). ANZCCART (1993). Euthanasia of animals used for scientific purposes. (J.S. Reilly, Ed.). ANZCCART, Australia.
Arslan M., Zaidi P., Lobo J., Zaidi A.A., and Qazi M.H. (1978a). Steroid levels in preovulatory and gravid lizards (Uromastix hardwicki). Gen. Comp. Endocrinol. 34 300-303.
Arslan M., Lobo J., Zaidi A.A., Jalali S. and Qazi M.H. (1978b). Annual androgen rhythm in the spiny-tailed lizard, Uromastix hardwicki. Gen. Comp. Physiol. 36 16-22.
Asahina K., Barry T.P., Aida K., Fusetani N. and Hanyu I. (1990). Biosynthesis of 17alpha, 20beta-dihydroxy-4-pregnen-3-one from 17alphahydroxyprogesterone by spermatozoa of the common carp, Cyprinus carpio. J. Exp. Zool. 255 244-249.
Balthazart J., Massa R. and Negri-Cesi P. (1979). Photoperiodic control of testosterone metabolism, plasma gonadotropins, cloacal gland growth, and reproductive behaviour in the Japanese quail. Gen. Comp. Endocrinol. 39 222-235.
Bauwens D., Van Damme R. and Verheyen R.F. (1989) Synchronization of spring molting with the onset of mating behaviour in male lizards, Lacerta vivipara. J. Herpetol. 23 89-91.
Bedrak E., Rosenstrauch A., Kafka M. and Friedlander M. (1983). Testicular steroidogenesis in the camel (Camelus dromedarius) during the mating and the nonmating seasons. Gen. Comp. Endocrinol. 52 255-264.
Belvedere P., Colombo L., Giacoma C., Malacarne G. and Andreoletti G.E. (1988). Comparative ethological and biochemical aspects of courtship pheromones in European newts. Monitore Zool. Ital. 22 397-403.
Bentley P.J. (1976). Comparative Vertebrate Endocrinology. Cambridge University Press, New York, USA.
Beuchat C.A. (1986). Reproductive influences on the thermoregulatory behavior of a live-bearing lizard. Copeia 1986 971-979.
Beuchat C.A. (1988). Temperature effects during gestation in a viviparous lizard. J. Therm. Biol. 13 135-142.
Blackburn D.G. (1998). Resorption of oviductal eggs and embryos in squamate reptiles. Herp. J. 8 65-71.
Blacker C., Akar A. and Scholler R. (1991). A simple technique for collecting blood of testicular origin: application to in vivo studies on testicular steroidogenesis in rats and Macaca fascicularis. J. Steroid Biochem. Molec. Biol. 39 105-114.
Bolaffi J.L. and Callard I.P. (1979). Plasma steroid profiles in male and female mudpuppies Necturus maculosus (Rafinesque). Gen. Comp. Endocrinol. 37 443-450.
Bolaffi J.L., Lance V., Callard I.P., Walsh J.M. and Idler D.R. (1979). Identification of 11-ketotestosterone, 11beta-hydroxytestosterone, and testosterone in plasma of Necturus maculosus (Rafinesque). Gen. Comp. Endocrinol. 38 127-131.
Bona-Gallo A., Licht P., MacKenzie D.S. and Lofts B. (1980). Annual cycles in levels of pituitary and plasma gonadotropin, gonadal steroid, and thyroid activity in the Chinese cobra (Naja naja). Gen. Comp. Endocrinol. 42 477-493.
Bonnet X. and Naulleau G. (1994). A body condition index (BCI) in snakes to study reproduction. Comptes Rendus de l'Academie des Sciences Serie III - Sciences de la Vie - Life Sciences. 317 34-41.
Bonnet X. and Naulleau G. (1996). Are body reserves important for reproduction in male dark green snakes (Colubridae: Coluber viridiflavus)? Herpetologica 52 137-146.
Bonnet X., Naulleau G. and Mauget R. (1994). The influence of body condition on 17beta-estradiol levels in relation to vitellogenesis in female Vipera aspis (Reptilia, Viperidae). Gen. Comp. Endocrinol. 93 424-437.
Borg B., Mayer I., Lambert J., Granneman J. and Schulz R. (1992). Metabolism of androstenedione and 11-ketotestosterone in the kidney of the three-spined stickleback, Gasterosteus aculeatus. Gen. Comp. Endocrinol. 86 248-256.
Boucek R.J. and Savard K. (1970). Steroid formation by the avian ovary in vitro (Gallus domesticus). Gen. Comp. Endocrinol. 15 6-11.
Bourne A.R. (1981). Blood metabolites of injected [14C]-progesterone in the lizard Tiliqua rugosa. Comp. Biochem. Physiol. 70B 661-664.
Bourne A.R. and Licht P. (1985). Steroid synthesis in turtle testes. Comp. Biochem. Physiol. 81B 793-796.
Bourne A.R. and Seamark R.F. (1972). Progestins in the plasma of a viviparous lizard, Tiliqua rugosa (Gray). J. Reprod. Fert. 28 156-157.
Bourne A.R. and Seamark R.F. (1973). The synthesis of corticosterone by the adrenal tissue of the lizard Tiliqua rugosa. Comp. Biochem. Physiol. 45B 275-277.
Bourne A.R. and Seamark R.F. (1975). Seasonal changes in 17beta-hydroxysteroids in the plasma of a male lizard (Tiliqua rugosa). Comp. Biochem. Physiol. 50B 535-536.
Bourne A.R. and Seamark R.F. (1978). Seasonal variation in steroid biosynthesis by the testis of the lizard Tiliqua nigrolutea. Comp. Biochem. Physiol. 59B 363-367.
Bourne A.R., Taylor J.L. and Watson T.G. (1985). Identification of epitestosterone in the plasma and testis of the lizard Tiliqua (Trachydosaurus) rugosa. Gen. Comp. Endocrinol. 58 394-401.
Bourne A.R., Taylor J.L. and Watson T.G. (1986a). Annual cycles of plasma and testicular androgens in the lizard Tiliqua (Trachydosaurus) rugosa. Gen. Comp. Endocrinol. 61 278-286.
Bourne A.R., Taylor J.L. and Watson T.G. (1986b). Effect of temperature on the seasonal production of testicular androgens, in vitro, by the lizard Tiliqua rugosa. Comp. Biochem. Physiol. 85A 527-530.
Bourne A.R., Stewart B.J. and Watson T.G. (1986c). Changes in blood progesterone concentration during pregnancy in the lizard Tiliqua (Trachydosaurus) rugosa. Comp. Biochem. Physiol. 84A 581-583.
Bradley A.J. (1990). Failure of glucocorticoid feedback during breeding in the male re-tailed phascogale Phascogale calura (Marsupialia: Dasyuridae). J. Steroid Biochem. 37 155-163.
Bradshaw S.D. and Fontaine-Bertrand E. (1970). Measurement of corticosteroids in reptilian and avian plasma by fluorometry and by competitive protein-binding radioassay. Comp. Biochem. Physiol. 36 37-48.
Buckmann D. (1987). Common origins and phylogenetic diversification of animal hormonal systems. In Development of Hormone Receptors. (G. Csaba, Ed.). pp155-166. Birkhausen Verlag, Basel and Boston.
Bull C.M. (1987). A population study of the viviparous Australian lizard, Trachydosaurus rugosus (Scincidae). Copeia 1987 749-757.
Bull C.M. (1990). Comparisons of displaced and retained partners in a monogamous lizard. Aust. Wildl. Res. 17 135-140.
Bull C.M. (1994). Population dynamics and pair fidelity in sleepy lizards. In Lizard Ecology - historical and experimental perspectives. (L.J. Vitt and E.R. Pianka, Eds). pp159-174. Princeton University Press, New Jersey.
Bull C.M. (1995). Population ecology of the sleepy lizard, Tiliqua rugosa, at Mt Mary, South Australia. Aust. J. Ecol. 20 393-402.
Bull C.M. and Pamula Y. (1996). Sexually dimorphic head sizes and reproductive success in the sleepy lizard Tiliqua rugosa. J. Zool. 240 511-521.
Bull C.M., McNally A. and Dubas G. (1991). Asynchronous seasonal activity of male and female sleepy lizards, Tiliqua rugosa. J. Herpetol. 25 436-441.
Bull C.M., Bedford G.S. and Schulz B.A. (1993a). How do sleepy lizards find each other? Herpetologica. 49 294-300.
Bull C.M., Pamula Y. and Schulze L. (1993b). Parturition in the sleepy lizard, Tiliqua rugosa. J. Herpetol. 27 489-492.
Bull J.J. and Shine R. (1979). Iteroparous animals that skip opportunities for reproduction. Amer. Nat. 114 296-303.
Callard G.V., Petro Z. and Ryan K.J. (1977). Identification of aromatase in the reptilian brain. Endocrinology 100 1214-1218.
Callard G.V., Petro Z. and Ryan K.J. (1980). Aromatization and 5alpha-reduction in brain and nonneural tissues of a cyclostome, Petromyzon marinus. Gen. Comp. Endocrinol. 42 155-159.
Callard I.P. (1967). Testicular steroid synthesis in the snake, Natrix sipedon pictiventris. J. Endocrinol. 37 105-106.
Callard I.P. and Ho S-m. (1987). Vitellogenesis and viviparity. In Fundamentals of Comparative Vertebrate Endocrinology. pp257-282. Plenum Press, New York.
Callard I.P. and Kleis S.M. (1987). Reproduction in reptiles. In Fundamentals of Comparative Vertebrate Endocrinology. pp187-205. Plenum Press, New York.
Callard I.P. and Klotz K.L. (1973). Sensitivity of parameters of estrogen action in the iguanid lizard Dipsosaurus dorsalis. Gen. Comp. Endocrinol. 21 314-321.
Callard I.P. and Leathem J.H. (1965). In vitro steroid synthesis by the ovaries of elasmobranchs and snakes. Arch. Anat. Microsc. 54 35-48.
Callard I.P. and Leathem J.H. (1966). Steroid synthesis by amphibian ovarian tissue. Gen. Comp. Endocrinol. 7 80-84.
Callard I.P., Doolittle J., Banks Jr. W.L. and Chan S.W. (1972a). Recent studies on the control of the reptilian ovarian cycle. Gen. Comp. Endocrinol. Suppl. 3 65-75.
Callard I.P., Chan S.W.C. and Potts, M.A. (1972b). The control of the reptilian gonad. Amer. Zool. 12 273-287.
Callard I.P., Bayne C.G. and McConnell W.F. (1972c). Hormones and reproduction in the female lizard Sceloporus cyanogenys. Gen. Comp. Endocrinol. 18 175-194.
Callard I.P., Lance V., Salhanick A.R. and Barad D. (1978). The annual ovarian cycle of Chrysemys picta: correlated changes in plasma steroids and parameters of vitellogenesis. Gen. Comp. Endocrinol. 35 245-257.
Callard I.P., Fileti L.A., Perez L.E., Sorbera L.A., Giannoukos G., Klosterman L.K., Tsang P. and McCracken J.A. (1992). Role of the corpus luteum and progesterone in the evolution of vertebrate viviparity. Amer. Zool. 32 264-275.
Camilleri C. and Shine R. (1990). Sexual dimorphism and dietary divergence: differences in trophic morphology between male and female snakes. Copeia 1990 649-658.
Cardone A., Angelini F. and Varriale B. (1998). Autoregulation of estrogen and androgen receptor mRNA and downregulation of androgen receptor mRNA by estrogen in primary cultures of lizard testis cells. Gen. Comp. Endocrinol. 110 227-233.
Carpenter C.C. (1978). Ritualistic social behavior in lizards. In Behavior and Neurology of Lizards (N. Greenberg and P.D. MacLean, Eds). pp253-267. NIMH.
Carolsfeld J., Tester M., Kreiberg H. and Sherwood N.M. (1997a). Pheromone-induced spawning of Pacific herring. I. Behavioral characterisation. Horm. Behav. 31 256-268.
Carolsfeld J., Tester M., Kreiberg H. and Sherwood N.M. (1997b). Pheromone-induced spawning of Pacific herring. II. Plasma steroids distinctive to fish responsive to spawning pheromone. Horm. Behav. 31 269-276.
Castilla A.M. and Swallow J.G. (1996). Thermal dependence of incubation duration under a cycling temperature regime in the lizard, Podarcis hispanica atrata. J. Herpetol. 30 247-253.
Castro A., Shih H.H.W. and Chung A. (1974). A simple radioimmunoassay of testosterone without column chromatography. Steroids 23 625-638.
Chan S.W.C. and Callard I.P. (1974). Reptilian ovarian steroidogenesis and the influence of mammalian gonadotropins (follicle-stimulating hormone and luteinizing hormone) in vitro. J. Endocr. 62 267-275.
Chan S.W.C., Ziegel S. and Callard I.P. (1973). Plasma progesterone in snakes. Comp. Biochem. Physiol. 44A 631-637.
Chieffi G. and Pierantoni R. (1987). Regulation of ovarian steroidogenesis. In Hormones and Reproduction in Fishes, Amphibians and Reptiles. (D.O. Norris and R.E. Jones, Eds). pp117-144. Plenum Press, New York.
Claus R. and Weiler U. (1994). Endocrine regulation of growth and metabolism in the pig: a review. Livestock Prod. Sci. 37 245-260.
Clausen H.J. (1940). Studies on the effect of ovariotomy and hypophysectomy on gestation in snakes. Endocrinology 27 700-704
Cobbetti A. and Zerani M. (1992). PGF-2-alpha, PGE-2, and sex steroids from the abdominal gland of the male crested newt Triturus carnifex (Laur). Prostaglandins 43 101-110.
Cogger H.G. (1992). Reptiles and Amphibians of Australia. Reed Books, Australia.
Cogger H.G. (1993). General description and definition of the class reptilia. In Amphibia and Reptilia. (C.J. Glasby, G.J.B. Ross and P.L. Beesley, Eds). Vol 2A, pp89-91. Australian Government Publishing Service, Canberra.
Colombo L., Lupo Di Prisco C. and Binder G. (1970). Metabolism of pregnenolone-4-14C by the testis of Gobius paganellus (Teleostei). Gen. Comp. Endocrinol. 15 404-419.
Cooke G.M. (1992). Phospholipases modulate immature pig testicular androgen and 16-androstene biosynthetic pathways in vitro. J. Steroid Biochem. Molec. Biol. 41 99-107.
Cooper W.E. Jr. (1994). Chemical discrimination by tongue-flicking in lizards: A review with hypotheses on its origin and its ecological and phylogenetic relationships. J. Chem. Ecol. 20 439-487.
Cooper W.E. Jr. (1995). Effects of estrogen and male head coloration on chemosensory investigation of female cloacal pheromones by male broad-headed skinks (Eumeces laticeps). Physiol. Behav. 58 1221-1225.
Cooper W.E. Jr. (1998). Prey chemical discrimination indicated by tongue-flicking in the eublepharis gecko Coleonyx varigatus. J. Exp. Zool. 281 21-25.
Cooper W.E. Jr. and Clarke R.F. (1982). Steroidal induction of the female reproductive coloration in the keeled earless lizard, Holbrookia propinqua. Herpetologica 38 425-429.
Cooper W.E. and Crews D. (1987). Hormonal induction of secondary sexual coloration and rejection behavior in female keeled earless lizards, Holbrookia propinqua. Anim. Behav. 35 1177-1187.
Cooper W.E. Jr. and Garstka W.R. (1987). Discrimination of male conspecific from male heterospecific odors by male scincid lizards (Eumeces laticeps). J. Exp. Zool. 241 253-256.
Cooper W.E. Jr. and Trauth S.E. (1992). Discrimination of conspecific male and female cloacal chemical stimuli by males and possession of a probable pheromone gland by females in a cordylid lizard, Gerrhosaurus nigrolineatus. Herpetologica 48 229-236.
Cooper W.E. Jr. and Vitt L.J. (1984a). Detection of conspecific odors by the female broad-headed skink, Eumeces laticeps. J. Exp. Zool. 229 49-54.
Cooper W.E. Jr. and Vitt L.J. (1984b). Conspecific odor detection by the male broad-headed skink, Eumeces laticeps: Effects of sex and site of odor source and of male reproductive condition. J. Exp. Zool. 230 199-209.
Cooper W.E. and Vitt L.J. (1986). Tracking of female conspecific odor trails by male broad-headed skinks (Eumeces laticeps). Ethology 71 242-248.
Cooper W.E. Jr., Mendonca M.T. and Vitt L.J. (1986a). Induction of sexual receptivity in the female broad-headed skink, Eumeces laticeps, by estradiol-17beta. Horm. Behav. 20 235-242.
Cooper W.E. Jr., Garstka W.R. and Vitt L.J. (1986b). Female sex pheromone in the lizard Eumeces laticeps. Herpetologica 42 361-366.
Courty Y. and Dufaure J.P. (1979). Levels of testosterone in the plasma and testis of the viviparous lizard (Lacerta vivipara Jaquin) during the annual cycle. Gen. Comp. Endocrinol. 39 336-342.
Courty Y. and Dufaure J.P. (1980). Levels of testosterone, dihydrotestosterone, and androstenedione in the plasma and testis of a lizard (Lacerta vivipara Jacquin) during the annual cycle. Gen. Comp. Endocrinol. 42 325-333.
Cree A. (1994). Low annual reproductive output in female reptiles from New Zealand. New Zeal. J. Zool. 21 351-372.
Cree A. and Guillette L.J. Jr. (1995). Biennial reproduction with a fourteen-month pregnancy in the gecko Hoplodactylus maculatus from southern New Zealand. J. Herpetol. 29 163-173.
Cree A., Guillette L.J., Cockrem J.F. and Joss J.M.P. (1990). Effects of capture and temperature stresses on plasma steroid concentrations in male tuatara (Sphenodon punctatus). J. Exp. Zool. 253 38-46.
Cree A., Cockrem J.F. and Guillette L.J. Jr. (1992). Reproductive cycles of male and female tuatara (Sphenodon punctatus) on Stephens Island, New Zealand. J. Zool., Lond. 226 199-217.
Crews D. (1975). Psychobiology of reptilian reproduction. Science 189 1059-1065.
Crews D. (1991). Trans-seasonal action of androgen in the control of spring courtship behavior in male red-sided garter snakes. Proc. Nat. Acad. Sci. USA. 88 3545-3548.
Crews D. and Gans C. (1992). The interaction of hormones, brain, and behavior: an emerging discipline in herpetology. In Biology of the Reptilia. (C. Gans and D. Crews, Eds). Vol 18, pp1-23. The University of Chicago Press, Chicago and London.
Crews D. and Morgentaler A. (1979). Effects of intracranial implantation of oestradiol and dihydrotestosterone on the sexual behaviour of the lizard Anolis carolinensis. J. Endocrinol. 82 373-381.
Crews D., Traina V., Wetzel F.T. and Muller C. (1978). Hormonal control of male reproductive behavior in the lizard, Anolis carolinensis: role of testosterone, dihydrotestosterone, and estradiol. Endocrinology 103 1814-1820.
Cuellar H.S., Roth J.J., Fawcett J.D. and Jones R.E. (1972). Evidence for sperm sustenance by secretions of the renal sexual segment of male lizards, Anolis carolinensis. Herpetologica 28 53-57.
Cuevas M.E., Miller W. and Callard G. (1992). Sulfoconjugation of steroids and the vascular pathway of communication in dogfish testis. J. Exp. Zool. 264 119-129.
Dale E. and Dorfman R.I. (1967). Conversion of progesterone-4-C14 to testosterone by testicular tissue of the American bullfrog. Gen. Comp. Endocrinol. 9 313-318.
Dauphin-Villemant C. and Xavier E. (1985). In vitro steroid biosynthesis by the adrenal gland of the female Lacerta vivipara Jacquin: the metabolism of exogenous precursors. Gen. Comp. Endocrinol. 58 1-9.
Dawson A. (1998). Natural and anthropogenic environmental oestrogens: the scientific basis for risk assessment. Comparative reproductive physiology of non-mammalian species. Pure Appl. Chem. 70 1657-1669.
Dehennin L. (1993). Secretion by the human testis of epitestosterone, with its sulfoconjugate and precursor androgen 5-androstene-3beta,17alpha-diol. J. Steroid Biochem. 44 171-177.
Delrio G., d'Istria M., Pierantoni R. and Fasano S. (1985). Identification and biosynthesis of androgens in non-mammalian vertebrates. In Current Trends in Comparative Endocrinology. (B. Lofts and W.N. Holmes, Eds). pp209-211. Hong Kong University Press, Hong Kong.
DeNardo D.F. and Sinervo G. (1994). Effects of steroid hormone interaction on activity and home-range size of male lizards. Horm. Behav. 28 273-287.
Depeiges A. and Dufaure J.P. (1982). Isolation and characterisation of a major soluble protein secreted by the epididymis of a nonmammalian vertebrate: immunohistochemical evidence of its binding to spermatozoa. Am. N. Y. Acad. Sci. 383 442-443.
Diaz J.A., Alonso-Gomez A.L. and Delgado M.J. (1994). Seasonal variation of gonadal development, sexual steroids, and lipid reserves in a population of the lizard Psammodromus algirus. J. Herpetol. 28 199-205.
Dubas G. and Bull C.M. (1992). Food addition and home range size of the lizard Tiliqua rugosa. Herpetologica 48 301-306.
Dufaure J.P. and Chambon M. (1978). Uptake of [3H]testosterone in several organs of the male viviparous lizard (Lacerta vivipara Jacquin) and selective retention by the epididymis. Gen. Comp. Endocrinol. 36 23-29.
Duggan R.T. and Lofts B. (1978). Steroid synthesis in the adrenal gland of the sea snake Hydrophis cyanocinctus: the metabolism of exogenous precursors. Gen. Comp. Endocrinol. 36 415-426.
Duke K.L. (1978). Nonfollicular ovarian components. In The Vertebrate Ovary. (R.E. Jones, Ed.). pp563-582. Plenum Press, New York.
Dunham A.E., Miles D.B. and Reznick D.N. (1988). Life history patterns in squamate reptiles. In Biology of the Reptilia. Ecology B. (C. Gans and R.B. Huey, Eds). Vol 16, pp421-515. Alan R. Liss Inc, New York.
Estrada-Flores E., Villagran-Santa Cruz M., Mendez-De La Cruz F. and Casa-Andeu G. (1990). Gonadal changes throughout the reproductive cycle of the viviparous lizard, Scleroporus mucronatus (Sauria: Iguanidae). Herpetologica 46 43-50.
Etches R.J. and Petitte J.N. (1990). Reptilian and avian follicular hierarchies: models for the study of ovarian development. J. Exp. Zool. Suppl. 4 112-122.
Evans R.M. (1988). The steroid and thyroid hormone receptor superfamily. Science 240 889-895.
Ferguson G.W. (1966). Releasers of courtship and territorial behaviour in the side blotched lizard Uta stansburiana. Anim. Behav. 15 89-92.
Ferguson G.W. (1970). Mating behaviour of the side-blotched lizards of the genus Uta (Sauria: Iguanidae). Anim. Behav. 18 65-72.
Fennell M.J. and Scanes C.G. (1992a). Effects of androgen (testosterone, 5alpha-dihydrotestosterone, 19-nortestosterone) administration on growth in turkeys. Poultry Science 71 539-547.
Fennell M.J. and Scanes C.G. (1992b). Inhibition of growth in chickens by testosterone, 5alpha-dihydrotestosterone, and 19-nortestosterone. Poultry Science 71 357-366.
Fergusson B. and Algar D. (1986). Home range and activity patterns of pregnant female skinks, Tiliqua rugosa. Aust. Wildl. Res. 13 287-294.
Fergusson B. and Bradshaw S.D. (1991). Plasma arginine vasotocin, progesterone, and luteal development during pregnancy in the viviparous lizard Tiliqua rugosa. Gen. Comp. Endocrinol. 82 140-151.
Fergusson B. and Bradshaw S.D. (1992). In vitro uterine contractions in the viviparous lizard Tiliqua rugosa: effects of gestation and steroid pretreatment in vivo. Gen. Comp. Endocrinol. 86 203-210.
Fergusson B., Bradshaw S.D. and Cannon J.R. (1985). Hormonal control of femoral gland secretion in the lizard, Amphibolurus ornatus. Gen. Comp. Endocrinol. 57 371-376.
Fevold H.R. and Eik-Nes K.B. (1963). Progesterone metabolism by testicular tissue of the English sparrow (Passer domesticus). Gen. Comp. Endocrinol. 3 335-345.
Flemming A.F. (1993a). Seasonal variation in testicular and fat-body weight and plasma testosterone and androstenedione concentration in the lizard Cordylus polyzonus (Sauria: Cordylidae). S. Afr. J. Zool. 28 127-131.
Flemming A.F. (1993b). The male reproductive cycle of the lizard Pseudocordylus. m. melanotus (Sauria: Cordylidae). J. Herpetol. 27 473-478.
Flemming A.F. (1993c). The female reproductive cycle of the lizard Pseudocordylus m. melanotus (Sauria: Cordylidae). J. Herpetol. 27 103-107.
Flores D.L. and Crews D. (1995). Effect of hormonal manipulation on sociosexual behavior in adult female leopard geckos (Eublepharis macularis), a species with temperature-dependent sex determination. Anim. Behav. 29 458-473.
Folman Y., Ahmad N., Sowell J.G. and Eik-Nes K.B. (1973). Formation in vitro of 5alpha-dihydrotestosterone and other 5alpha-reduced metabolites of 3H-testosterone by the seminiferous tubules and interstitial tissue from immature and mature rat testes. Endocrinology 92 41-47.
Fox H. (1977). The urogenital system of reptiles. In Biology of the Reptilia. (C. Gans, Ed.). Vol 6, pp1-157. Academic Press, London and New York.
Frieden E. and Lippner H. (1971). Biochemical endocrinology of the vertebrates. Prentice-Hall, New Jersey, USA.
Galena H.J., Pillai A.K. and Terner C. (1974). Progesterone and androgen receptors in non-flagellate germ cells of the rat testis. J. Endocrinol. 63 223-237.
Gavaud J. (1986). Vitellogenesis in the lizard Lacerta vivipara Jacquin. 2. Vitellogenin synthesis during the reproductive cycle and its control by ovarian steroids. Gen. Comp. Endocrinol. 63 11-23.
Gavaud J. (1983). Obligatory hibernation for completion of vitellogenesis in the lizard Lacerta vivipara J. J. Exp. Zool. 225 397-405.
Gemmel R.T. (1995). A comparative study of the corpus luteum. Reprod. Fertil. Dev. 7 303-312.
Giannoukos G. and Callard I.P. (1995). Reptilian (Chrysemys picta) hepatic progesterone-receptors: relationship to plasma steroids and the vitellogenic cycle. J. Steroid. Biochem. Molec. Biol. 55 93-106.
Girling J.E., Cree A. and Guillette L.J. Jr. (1998). Oviducal structure in four species of gekkonid lizard differing in parity mode and eggshell structure. Reprod. Fert. Dev. 10 139-154.
Gist D.H. and Congdon J.D. (1998). Oviductal sperm storage as a reproductive tactic of turtles. J. Exp. Zool. 282 526-534.
Gist D.H. and DeRoos R. (1966). Corticoids of the alligator adrenal gland and the effects of ACTH and progesterone on their production in vitro. Gen. Comp. Endocrinol. 7 304-313.
Gobbetti A. and Zerani M. (1995). A novel neuropeptide cellular mechanism in amphibian interrenal steroidogenesis. Cell. Signalling 7 269-275.
Gobbetti A., Zerani M., Di Fiore M.M. and Botte V. (1994). Relationships among GnRH, substance P, prostaglandins, sex steroids and aromatase activity in the brain of the male lizard Podarcis sicula sicula during reproduction. J. Reprod. Fert. 101 523-529.
Godwin J., Hartman V.M., Grammer M. and Crews D. (1996). Progesterone inhibits female-typical receptive behaviour and decreases hypothalamic estrogen and progesterone receptor messenger ribonucleic acid levels in whiptail lizards (Genus Cnemidophorus). Horm. Behav. 30 138-144.
Goldberg S.R. (1971). Reproductive cycles of the ovoviviparous iguanid lizard Sceloporus jarrovi Cope. Herpetologica 27 123-131.
Goldberg S.R. and Bezy R.L. (1974). Reproduction in the island night lizard, Xantusia riversiana. Herpetologica 30 350-360.
Graves B.M. and Halpern M. (1991). Discrimination of self from conspecific chemical cues in Tiliqua scincoides (Sauria: Scincidae). J. Herpetol. 25 125-126.
Green D. (1995). A comparison of three litters in the shingleback lizard, Trachydosaurus rugosus. Herpetofauna 25 42-43.
Greenberg N. and Crews D. (1990). Endocrine and behavioral responses to aggression and social dominance in the green anole lizard, Anolis carolinensis. 77 246-255.
Greer A.E. (1989). The Biology and Evolution of Australian Lizards. Surrey Beatty and Sons, Pty, Ltd, Australia.
Guarino F.G., Paulesu L., Cardone A., Bellini L., Ghiara G. and Angelini F. (1998). Endocrine activity of the corpus luteum and placenta during pregnancy in Chalcides chalcides (Reptilia, Squamata). Gen. Comp. Endocrinol. 111 261-270.
Guillette L.J. Jr. (1982). The evolution of viviparity and placentation in the high elevation, Mexican lizard, Scleroporus aeneus. Herpetologica 38 94-103.
Guillette L.J. Jr. and Jones R.E. (1985). Ovarian, oviducal, and placental morphology of the reproductively bimodal lizard, Sceloporus aeneus. J. Morphol. 184 85-98.
Guillette L.J. Jr., Spielvogel S. and Moore F.L. (1981). Luteal development, placentation, and plasma progesterone concentration in the viviparous lizard Sceloporus jarrovi. Gen. Comp. Endocrinol. 43 20-29.
Guillette L.J. Jr., Demarco V. and Palmer B.D. (1991). Exogenous progesterone or indo-methacin delays parturition in the viviparous lizard Sceloporus jarrovi. Gen. Comp. Endocrinol. 81 105-112.
Guillette L.J. Jr., Cree A. and Rooney A.A. (1995) Biology of stress: interactions with reproduction, immunology and intermediary metabolism. In Health and Welfare of Captive Reptiles. (C. Warwick, F.L. Frye and J.B. Murphy, Eds). pp32-81. Chapman and Hall, London.
Guillette L.J. Jr., Woodward A.R., Crain D.A., Masson G.R., Palmer B.D., Cox S.M., You-Xiang Q. and Orlando E.F. (1997). The reproductive cycle of the female American alligator (Alligator mississippiensis). Gen. Comp. Endocrinol. 108 87-101
Halpert A.P., Garstka W.R. and Crews D. (1982). Sperm transport and storage and its relation to the annual sexual cycle of the female red-sided garter snake, Thamnophis sirtalis parietalis. J. Morphol. 174 149-159.
Hay J.B., Hodgins M.B. and Roberts R.J. (1976). Androgen metabolism in skin and skeletal muscle of the rainbow trout (Salmo gairdnerii) and in accessory sexual organs of the spur dogfish (Squalus acanthias). Gen. Comp. Endocrinol. 29 402-413.
Heatwole H. and Taylor J. (1987). Reproductive ecology. In Ecology of Reptiles. (H. Heatwole Ed.). pp147-166. Surrey Beatty and Sons Pty Ltd, N.S.W., Australia.
Heistermann M., Tari S. and Hodges J.K. (1993). Measurement of fecal steroids for monitoring ovarian function in new world primates, Callitrichidae. J. Reprod. Fertil. 99 243-251.
Henle K. (1990). Notes on the population ecology of the large herbivorous lizard, Trachydosaurus rugosus, in arid Australia. J. Herpetol. 24 100-103.
Herrel A., Van Damme R. and De Vree F. (1996). Sexual dimorphism of head size in Podarcis hispanica atrata: testing the dietary divergence hypothesis by bite force analysis. Neth. J. Zool. 46 253-262.
Hews D.K. (1990). Examining hypotheses generated by field measures of sexual selection on male lizards, Uta palmeri. Evolution 44 1956-1966.
Hews E.A. and Kime D.E. (1978). Testicular steroid biosynthesis by the green lizard Lacerta viridis. Gen. Comp. Endocrinol. 35 432-435.
Hews D.K. and Moore M.C. (1995). Influence of androgens on differentiation of secondary sex characters in tree lizards, Urosaurus ornatus. Gen. Comp. Endocrinol. 97 86-102.
Hews D.K., Knapp R. and Moore M.C. (1994). Early exposure to androgens affects adult expression of alternative male types in tree lizards. Horm. Behav. 28 96-115.
Highfill D.R. and Mead R.A. (1975a). Sources and levels of progesterone during pregnancy in the garter snake, Thamnophis elegans. Gen. Comp. Endocrinol. 27 389-400.
Highfill D.R. and Mead R.A. (1975b). Function of corpora lutea in the viviparous garter snake, Thamnophis elegans. Gen. Comp. Endocrinol. 27 401-407.
Ho S.-m. (1987). Endocrinology of vitellogenesis. In Hormones and Reproduction in Fishes, Amphibians and Reptiles. (D.O. Norris and R.E. Jones, Eds). pp145-169. Plenum Press, New York.
Ho. S.-m., Kleis S., McPherson R., Heisermann G.J. and Callard I.P. (1982). Regulation of vitellogenesis in reptiles. Herpetologica 38 40-50.
Hoschoian J.C., Cardoso M.E.L., Coumroglon D.M. and Andrada J.A. (1991). Biosynthesis of testicular steroids in the immature, adult and senescent guinea-pig. J. Steroid Biochem. Molec. Biol. 38 233-240.
Huang D.P., Vinson G.P. and Phillips J.G. (1969). The metabolism of pregnenolone and progesterone by cobra adrenal tissue in vitro and the effects of ACTH on product yield time curves. Gen. Comp. Endocrinol. 12 637-643.
Huang F.-L., Liu T.-C. and Lo T.-B. (1985). Biosynthesis of 11-ketotestosterone by carp testis in vitro. In Current Trends in Comparative Endocrinology. (B. Lofts and W.N. Holmes, Eds). pp233-234. Hong Kong University Press, Hong Kong.
Huf P.A., Bourne A.R. and Watson T.G. (1987a). Metabolism of androgens in the brain of the lizard Tiliqua rugosa in vitro. In Proc. Aust. Soc. Reprod. Biol. p79. 19th annual conference, Sydney, Australia.
Huf P.A., Bourne A.R. and Watson T.G. (1987b). Identification of testosterone sulfate in the plasma of the male lizard Tiliqua rugosa. Gen. Comp. Endocrinol. 66 364-368.
Huf P.A., Bourne A.R. and Watson T.G. (1989). The in vitro biosynthesis of epitestosterone and testosterone from C19 steroid precursors in the testis of the lizard Tiliqua rugosa. Gen. Comp. Endocrinol. 75 280-286 .
Hutchinson M.N., Robertson P. and Rawlinson P.A. (1989). Redescription and ecology of the endemic Tasmanian scincid lizards Leiolopisma microlepidotum and L. pretiosum. Pap. Proc. R. Soc. Tasm. 123 257-274.
Ibarguengoytia N.R. and Cussac V.E. (1996). Reproductive biology of the viviparous lizard, Liolaemus pictus (Tropiduridae) - biennial female reproductive cycle. Herp. J. 6 137-143.
Ibarguengoytia N.R. and Cussac V.E. (1998). Reproduction of the viviparous lizard Liolaemus elongatus in the highlands of southern South America: plastic cycles in response to climate? Herp. J. 8 99-105.
Idler D.R., Horne D.A. and Sangalang G.B. (1971). Identification and quantification of the major androgens in testicular and peripheral plasma of Atlantic salmon (Salmo salar) during sexual maturation. Gen. Comp. Endocrinol. 16 257-267.
Ina P.G. (1991). Quantitative review on the use of anabolic hormones in ruminants for meat production. I. Animal performance. Annal. De Zootech. 42 333-359.
Inano H., Tsuno K. and Tamaoki B. (1970). Identification of 7alpha-hydroxylated androgens as the metabolites of androstenedione by testicular microsomal fraction of rats. Biochemistry 9 2253-2260.
Johnson L.F., Jacob J.S. and Torrance P. (1982). Annual testicular and androgenic cycles of the cottonmouth (Agkistrodon piscivorus) in Alabama. Herpetologica 38 16-25.
Johnson M.H. and Everitt B.J. (1988). Essential Reproduction. 3rd ed. Blackwell Scientific Publications, Great Britain.
Jones R.E. (1991). Human Reproductive Biology. Academic press, Inc, USA.
Jones R.E. and Guillette L.J. Jr. (1982). Hormonal control of oviposition and parturition in lizards. Herpetologica 38 80-93.
Jones S.M. and Rose R.W. (1992). Plasma progesterone levels in the pregnant female rat kangaroo (Bettongia gaimardi). Gen. Comp. Endocrinol. 87 178-182.
Jones S.M. and Swain R. (1996). Annual reproductive cycle and annual cycles of reproductive hormones in plasma of female Niveoscincus metallicus (Scincidae) from Tasmania. J. Herpetol. 30 140-146.
Jones S.M., Wapstra E. and Swain R. (1997). Asynchronous male and female gonadal cycles and plasma steroid concentrations in a viviparous lizard, Niveoscincus ocellatus (Scincidae) from Tasmania. Gen. Comp. Endocrinol. 108 271-281.
Joss J.M.P. (1985). Ovarian steroid production in oviparous lizards of the genus Lampropholis (Scincidae). In The Biology of Australasian Frogs and Reptiles (G Grigg, R Shine and H Ehmann, Eds). pp 319-326. Royal Zoological Society of New South Wales, Australia.
Joss J.M.P. and Minard J.A. (1985). On the reproductive cycles of Lampropholis guichenoti and L. delicata (Squamata: Scincidae) in the Sydney region. Aust. J. Zool. 33 699-704.
Joss J.M.P., Edwards A. and Kime D.E. (1996). In vitro biosynthesis of androgens in the Australian lungfish, Neoceratodus forsteri. Gen. Comp. Endocrinol. 101 256-263.
Kemppainen J.A., Lane M.V., Sar M. and Wilson E.M. (1992). Androgen receptor phosphorylation, turnover, nuclear transport, and transcriptional activation: specificity for steroids and antihormones. J. Biol. Chem. 267 968-974.
Kime D.E. (1979). The effect of temperature on the testicular steroidogenic enzymes of the rainbow trout, Salmo gairdneri. Gen. Comp. Endocrinol. 39 290-296.
Kime D.E. (1980). Androgen biosynthesis by the testes of the goldfish Carassius auratus in vitro: the effect of temperature on the formation of steroid glucuronides. Gen. Comp. Endocrinol. 41 164-172.
Kime D.E. (1987). The steroids. In Fundamentals of Comparative Vertebrate Endocrinology. (I. Chester-Jones, P. M. Ingleton and J. G. Philips, Eds). pp3-56. Plenum Press, New York.
Kime D.E. (1990). In vitro metabolism of progesterone, 17-hydroxyprogesterone, and 17, 20beta-dihydroxy-4-pregnen-3-one by ovaries of the common carp Cyprinus carpio: production rates of polar metabolites. Gen. Comp. Endocrinol. 79 406-414.
Kime D.E. and Abdullah M.A.S. (1994). The in vitro metabolism of 17-hydroxyprogesterone by the ovaries of the goldfish, Carassius auratus, is affected by substrate concentration. Gen. Comp. Endocrinol. 95 109-116.
Kime D.E. and Callard G.V. (1982). Formation of 15alpha-hydroxylated androgens by the testis and other tissues of the sea lamprey, Petromyzon marinus, in vitro. Gen. Comp. Endocrinol. 4 267-270.
Kime D.E. and Ebrahimi M. (1997). Synthesis of 17, 20alpha- and 17, 20beta-dihydroxy-4-pregnen-3-ones, 11-ketotestosterone and their conjugates by gills of teleost fish. Fish Physiol. Biochem. 17 117-121.
Kime D.E. and Groves D.J. (1986). Steroidogenesis by gonads of a viviparous teleost, the sailfin molly (Poecilia latipinna), in vitro and in vivo. Gen. Comp. Endocrinol. 63 125-133.
Kime D.E. and Hews E.A. (1978a). In vitro biosynthesis of 11beta-hydroxy- and 11-oxotestosterone by testes of the pike (Esox lucius) and the perch (Perca fluviatilis). Gen. Comp. Endocrinol. 36 604-608.
Kime D.E. and Hews E.A. (1978b). Androgen biosynthesis in vitro by testes from amphibia. Gen. Comp. Endocrinol. 35 280-288.
Kime D.E. and Hews E.A. (1980). Steroid biosynthesis by the ovary of the hagfish Myxine glutinosa. Gen. Comp. Endocrinol. 42 71-75.
Kime D.E. and Hews E.A. (1982). The effect of temperature on steroid biosynthesis by testes of the dogfish, Scyliorhinus caniculus. Comp. Biochem. Physiol. 71B 675-679.
Kime D.E. and Hyder M. (1983). The effect of temperature and gonadotropin on testicular steroidogenesis in Sarotherodon (Tilapia) mossambicus in vitro. Gen. Comp. Endocrinol. 50 105-115.
Kime D.E. and Rafter J.J. (1981). Biosynthesis of 15-hydroxylated steroids by gonads of the river lamprey, Lampetra fluviatilis, in vitro. Gen. Comp. Endocrinol. 44 69-76.
Kime D.E. and Saksena D.N. (1980). The effect of temperature on the hepatic catabolism of testosterone in the rainbow trout (Salmo gairdneri) and the goldfish (Carassius auratus). Gen. Comp. Endocrinol. 42 228-234.
Kime D.E., Hews E.A. and Rafter J. (1980). Steroid biosynthesis by testes of the hagfish Myxine glutinosa. Gen. Comp. Endocrinol. 41 8-13.
Krohmer R.H. and Aldridge R.D. (1985). Female reproductive cycle of the lined snake (Tropidoclonion lineatum). Herpetologica 41 39-44.
Krohmer R.W., Grassman M. and Crews D. (1987). Annual reproductive cycle in the male red-sided garter snake, Thamnophis sirtalis parietalis: field and laboratory studies. Gen. Comp. Endocrinol. 68 64-75.
Kleis-San Francisco S. and Callard I.P. (1986). Progesterone receptors on the oviduct of a viviparous snake (Nerodia): correlations with ovarian function and plasma steroid levels. Gen. Comp. Endocrinol. 63 220-229.
Kobayashi T, Sakai N., Adachi S., Asahina K., Iwasawa H and Nagahama Y. (1993). 7alpha-20alpha-Dihydroxy-4-pregnen-3-one is the naturally occurring spermiation-inducing hormone in the testis of a frog, Rana nigromaculata. Endocrinology 133 321-327.
Koelle M.R., Talbot W.S., Segraves W.A., Bender M.T., Cherbas P. and Hogness D.S. (1991). The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily. Cell 67 59-77.
Kreger M.D. and Mencsh J.A. (1993). Physiological and behavioral effects of handling and restraint in the ball python (Python regius) and the blue-tongued skink (Tiliqua scincoides). Appl. Anim. Behav. Sci. 38 323-336.
Kuipers H., Wijnen J.A.G., Hartgens F. and Willems S.M.M. (1991). Influence of anabolic steroids on body composition, blood pressure, lipid profile and liver functions in body builders. Internat. J. Sports Med. 12 413-418.
Lance V. (1984). Endocrinology of reproduction in male reptiles. Symp. Zool. Soc. Lond. 52 357-383.
Lance V. and Callard I.P. (1978). Hormonal control of ovarian steroidogenesis in nonmammalian vertebrates. In The Vertebrate Ovary. (R.E. Jones, Ed.) pp361-407. Plenum Press, New York.
Lance V.A. and Elsey R.M. (1986). Stress-induced suppression of testosterone secretion in male alligators. J. Exp. Zool. 239 241-246.
Larsen K.W., Gregory P.T. and Antoniak R. (1993). Reproductive ecology of the common garter snake Thamnophis sirtalis at the northern limit of its range. Am. Midl. Nat. 129 336-345.
Laudet V., Hanni C., Coll J., Catzeflis F. and Stehlin D. (1992). Evolution of the nuclear receptor gene superfamily. EMBO J. 11 1003-1013.
LeBreton M. (1990). Reproductive notes on the eastern blue-tongue lizard Tiliqua scincoides (White 1790). Herpetofauna 20 30-32.
Leitz T. and Reinboth R. (1987). The biosynthesis of 11-ketotestosterone by the testis of the Siamese fighting fish Betta splendens Regan (Anabantoidei, Belontiidae). Gen. Comp. Endocrinol. 66 145-157.
Leloup-Hatey J., Oudinet J.P. and Lopez E. (1985). Testicular steroidogenesis during gonadotropin-induced spermatogenesis in male European eel (Anguilla anguilla L.). In Current Trends in Comparative Endocrinology. (B. Lofts and W.N. Holmes, Eds). pp229-232. Hong Kong University Press, Hong Kong.
LeRoith D., Delahunty G., Wolson G.L., Roberts C.T. Jr., Shemer J., Hart C., Lesniak M.A., Shiloach J. and Roth J. (1986). Evolutionary aspects of the endocrine and nervous systems. Recent Prog. Horm. Res. 42 549-587.
Liley N.R. (1982). Chemical communication in fish. Can. J. Fish. Aquat. Sci. 39 22-35.
Lindzey J. and Crews D. (1986). Hormonal control of courtship and copulatory behaviour in male Cnemidophorous inornatus, a direct sexual ancestor of a unisexual, parthenogenetic lizard. Gen. Comp. Endocrinol. 64 411-418.
Lindzey J. and Crews D. (1988). Effects of progestins on sexual behaviour in castrated lizards (Cnemidophorus inornatus). J. Endocr. 119 265-273.
Lindzey J. and Crews D. (1992). Interactions between progesterons and androgens in the stimulation of sex behaviours in male little striped whiptail lizards, Cnemidophorus inornatus. Gen. Comp. Endocrinol. 86 52-58.
Lofts B. (1972). The sertoli cell. Gen. Comp. Endocrinol. Suppl. 3 636-648.
Lofts B. (1987). Testicular function. In Hormones and Reproduction in Fishes, Amphibians and Reptiles. (D.O. Norris and R.E. Jones, Eds). pp283-325. Plenum Press, New York.
Lofts B. and Choy L.Y.L. (1971). Steroid synthesis by the seminiferous tubules of the snake Naja naja. Gen. Comp. Endocrinol. 17 588-591.
Lombardo M.E., Hakky S.I., Hall M.K. and Hudson P.B. (1993). A study of androgen biosynthesis by the human testis in vitro. J. Steroid Biochem. 44 191-198.
Lupo Di Prisco C., Delrio G. and Chieffi G. (1968). Sex hormones in the ovaries of the lizard Lacerta sicula. Gen. Comp. Endocrinol. 10 292-295.
Lupo Di Prisco C., Delrio G., Chieffi G., Cardellini L.B. and Magni A.P. (1971). Identification and biosynthesis of steroid hormones in the ovary and fat bodies of female Triturus cristatus carnifex. Comp. Biochem. Physiol. 40B 53-60.
Lupo Di Prisco C., Baslie C., Delrio G. and Chieffi G. (1972). In vitro metabolism of cholesterol-4-14C and testosterone-4-14C in testes and fat bodies of Triturus cristatus carnifex. Comp.Biochem. Physiol. 41B 245-249.
Maderson P.F.A. and Chiu K.W. (1985). Endocrine relationships of secondary sexual derivatives in tetrapods. In Current Trends in Comparative Endocrinology (B. Lofts and W.N. Holmes, Eds). pp 1191-1194. Hong Kong University Press, Hong Kong.
Mak P., Callard I.P. and Callard G.V. (1983). Characterization of an estrogen receptor in the testis of the urodele amphibian Necturus maculosus. Biol. Reprod. 28 261-270.
Manning N.J. and Kime D.E. (1985). The effect of temperature on testicular steroid production in the rainbow trout, Salmo gairdneri, in vivo and in vitro. Gen. Comp. Endocrinol. 57 377-382.
Marion K.R. (1982). Reproductive cues for gonadal development in temperate reptiles: temperature and photoperiod effects on the testicular cycle of the lizard Sceloporus undulatus. Herpetologica 38 26-39.
Martins E.P. (1994). Structural complexity in a lizard communication system: the Sceloporus graciosus "push-up" display. Copeia 1994 994-955.
Masson G.R. and Guillette L.J. Jr. (1987). Changes in oviducal vascularity during the reproductive cycle of three oviparous lizards (Eumeces obsoletus, Sceloporus undulatus and Crotaphytus collaris). J. Reprod. Fert. 80 361-371.
Mathies T. and Andrews R.M. (1995). Thermal and reproductive biology of high and low elevation populations of the lizard Sceloporus scalaris: implications for the evolution of viviparity. Oecologica 104 101-111.
McDonald I.R., Lee A.K., Than K.A. and Martin R.W. (1988). Concentration of free glucocorticoids in plasma and mortality in the Australian bush rat (Ruttus fuscipes Waterhouse). J. Mamm. 69 740-748.
McKinney R.B. and Marion K.R. (1985). Plasma androgens and their association with the reproductive cycle of the male fence lizard, Scleroporus undulatus. Comp. Biochem. Physiol. 82A 515-519.
McMurray J. (1988). Organic Chemistry. Brooks/Cole Publishing Company, California.
McNicol D. Jr. and Crews D. (1979). Estrogen/progesterone synergy in the control of female sexual receptivity in the lizard, Anolis carolinensis. Gen. Comp. Endocrinol. 38 68-74.
Mead R.A., Eroschenko V.P. and Highfill D.R. (1981). Effects of progesterone and estrogen on the histology of the oviduct of the garter snake, Thamnophis elegans. Gen. Comp. Endocrinol. 45 345-354.
Melville J. and Swain R. (1999). Home-range characteristics of an alpine lizard. Niveoscincus microlepidotus (Scincidae), on Mt Wellington, southern Tasmania. Wildl. Res. 26 263-270.
Mendez-De la Cruz F.R., Villagran-Santa Cruz M. and Andrews R.M, (1998). Evolution of viviparity in the lizard genus Sceloporus. Herpetologica 54 521-532.
Mendonca M.T., Tousibnant A.J. and Crews D. (1996). Pinealectomy, melatonin, and courtship behaviour in male red-sided garter snakes (Thamnophis sirtalis parietalis). J. Exp. Zool. 274 63-74.
Milewich L., Whisenant M.G. and Sawyer M.K. (1982). Androstenedione metabolism by human lymphocytes. J. Steroid Biochem. 16 81-85.
Moore F.L. (1987). Regulation of reproductive behaviors. In Hormones and Reproduction in Fishes, Amphibians and Reptiles. (D.O. Norris and R.E. Jones, Eds). pp505-522. Plenum Press, New York.
Moore M.C. (1986). Elevated testosterone levels during non-breeding season territoriality in a fall-breeding lizard, Sceloporus jarrovi. J. Comp. Physiol. 158A 159-163.
Moore M.C. and Crews D. (1986). Sex steroid hormones in natural populations of a sexual whiptail lizard Cnemidophorus inornatus, a direct evolutionary ancestor of a unisexual parthenogen. Gen. Comp. Endocrinol. 63 424-430.
Moore M.C. and Lindzey J. (1992). The physiological basis of sexual behavior in male reptiles. In Biology of the Reptilia. Physiology E. (C. Gans and D. Crews, Eds). Vol 18. pp70-113. The University of Chicago Press, Chicago and London.
Moore M.C., Whittier J.M. and Crews D. (1985a). Sex steroid hormones during the ovarian cycle of an all-female, parthenogenic lizard and their correlation with pseudosexual behaviour. Gen. Comp. Endocrinol. 60 144-153.
Moore M.C., Whittier J.M., Billy A.J. and Drews D. (1985b). Male-like behaviour in an all female lizard: relationship to ovarian cycle. Anim. Behav. 33 284-289.
Moore M.C., Thompson C.W. and Marler C.A. (1991). Reciprocal changes in corticosterone and testosterone levels following acute and chronic handling stress in the tree lizard, Urosaurus ornatus. Gen. Comp. Endocrinol. 81 217-226.
Morel L. Dufaure J.P, and Depeiges A. (1993). LESP, an androgen-regulated lizard epithelial secretory protein family identified as a new member of the lipocalin superfamily. J. Biol. Chem. 268 10274-10281.
Motta C.M., Castriota Scandenberg M., Filosa S. and Andreuccetti. (1995). Role of pyriform cells during the growth of oocytes in the lizard Podarcis sicula. J. Exp. Zool. 273 247-256.
Mouton P.Le F.N., and Van Wyk J.H. (1993). Sexual dimorphism in cordylid lizards: a case study of the Drakensberg crag lizard, Pseudocordylus melanotus. Can. J. Zool. 71 1715-1723.
Naulleau G. and Fleury F. (1990). Changes in plasma progesterone in female Vipera aspis L. (Reptilia, Viperidae) during the sexual cycle in pregnant and nonpregnant females. Gen. Comp. Endocrinol. 78 433-443.
N'Diaye A., Sandoz D., Boisvieux-Ulrich E. and Ozon R. (1974). Action des androgenes chez l'amphibien anoure Discoglossus pictus (Otth). III. Effets de la castration et action des hormones androgenes sur les ultrastructures de la vesicule seminale. Gen. Comp. Endocrinol. 24 286-304.
Neaves W.B. (1970). Intracellular bridges between follicle cells and oocyte in the lizard, Anolis carolinensis. Anat. Rec. 170 285-302.
Nilson G. (1980). Male reproductive cycle of the European adder, Vipera berus, and its relation to annual activity periods. Copeia 1980 729-737.
Nilson G. and Andren C. (1982). Function of renal sex secretion and male hierarchy in the adder, Vipera berus, during reproduction. Horm. Behav. 16 404-413.
Norris D.O. (1987). Regulation of male gonaducts and sex accessory structures. In Hormones and Reproduction in Fishes, Amphibians and Reptiles. (D.O. Norris and R.E. Jones, Eds). pp327-354. Plenum Press, New York.
Norris D.O. (1997). Vertebrate Endocrinology. Third edition. Academic Press, Inc, USA.
Olsson M. and Shine R. (1997). The seasonal timing of oviposition in sand lizards (Lacerta agilis) - why early clutches are better. J. Evol. Biol. 10 369-381.
Owen F.J., Cake M.H. and Bradshaw S.D. (1982). Characterisation and properties of a progesterone receptor in the uterus of the quokka (Setonix brachyurus). J. Endocrinol. 93 17-24.
Owens D.W. (1997). Hormones in the life history of sea turtles. In The Biology of Sea Turtles. (P.L. Lutz and J.A. Musick, Eds). pp315-314. CRC Press, Inc, Boca Raton, New York, London, Tokyo.
Ozon R. (1967). Synthese, in vitro des hormones steroides dans le testicule et l'ovaire de l'Ambhibien Urodele Pleurodeles waltlii Michah. Gen. Comp. Endocrinol. 8 214-227.
Ozon R. (1972). Androgens in fishes, amphibians, reptiles and birds. In Steroids in Nonmammalian Vertebrates (D.R. Idler, ed.), pp328-389, Academic Press, New York and London.
Ozon R and Breuer H. (1964). Untersuchungen uber den Stoffwechsel von Steroidhormonen bei Vertebraten, IV. Aromatisierung von Testosteron zu Ostrogenen im Ovar des Frosches (Rana temporaria). Z. Physiol. Chem. 337 61-65.
Ozon R. and Breuer H. (1966). Studies on the metabolism of steroid hormones in vertebrates VII. Enzymatic glucuronidation of estrogens by Pleurodeles waltlii Micah (Amphibia: Urodela) in vivo and in vitro. Gen. Comp. Endocrinol. 6 295-302.
Ozon R. and Fouchet C. (1972). Action des androgenes chez l'Amphibien Anoure Discoglossus pictus I. Metabolisme in vitro de la testosterone par les vesicules seminales. Gen. Comp. Endocrinol. 19 484-493.
Ozon R. and Stocker C. (1974). Formation in vitro de 5alpha-dihydrotestosterone par le testicule de Discoglossus pictus. Gen. Comp. Endocrinol. 23 224-236.
Palmer B.D. and Guillette L.J.Jr. (1988). Histology and functional morphology of the female reproductive tract of the tortoise Gopherus polyphemus. An. J. Anat. 183 200-211.
Palmer B.D., Demarco V.G. and Guillette L.J.Jr. (1993). Oviductal morphology and eggshell formation in the lizard, Sceloporus woodi. J. Morphol. 217 205-217.
Paolucci M. and Di Fiore M.M. (1994). Estrogen and progesterone receptors in lizard Podarcis s. sicula oviduct: seasonal distribution and hormonal dependence. J. Exp. Zool. 269 432-441.
Paolucci M., Di Fiore M.M., Ciarcia G. and Botte V. (1992). Plasma sex steroid binding proteins (SSBP) in the male lizard, Podarcis s. sicula, during the reproductive cycle. Gen. Comp. Endocrinol. 87 232-239.
Patterson J.W. (1983). Frequency of reproduction, clutch size and clutch energy in the lizard Anguis fragilis. Amphibia-Reptilia 4 195-203.
Payne A.H. (1980). Testicular steroid sulfotransferases: comparison to liver and adrenal steroid sulfotransferases of the mature rat. Endocrinology. 106 1365-1370.
Perkins M.J. and Palmer B.D. (1996). Histology and functional morphology of the oviduct of an oviparous snake, Diadophis punctatus. J. Morphol. 227 67-79.
Pesonen M., Hansson T., Foflin L. and Andersson T. (1990). Regional distribution of microsomal xenobiotic and steroid metabolism in kidney microsomes from rainbow trout. Fish Physiol. Biochem. 8 141-145.
Pottinger T.G. and Pickering A.D. (1985). The effects of 11-ketotestosterone and testosterone on the skin structure of brown trout, Salmo trutta L. Gen. Comp. Endocrinol. 59 335-342.
Prasad M.R.N. and Reddy P.R.K. (1972). Physiology of the sexual segment of the kidney in reptiles. Gen. Comp. Endocrinol. 3 649-662.
Prasad M.R.N. and Sanyal M.K. (1969). Effect of sex hormones on the sexual segment of kidney and other accessory reproductive organs of the Indian house lizard Hemidactylus flaviviridis Ruppell. Gen. Comp. Endocrinol. 12 110-118.
Prestt I. (1971). An ecological study of the viper Vipera berus in southern Britain. J. Zool. Lond. 164 373-418.
Qualls C.P. and Shine R. (1995). Maternal body-volume as a constraint on reproductive output in lizards - evidence from the evolution of viviparity. Oecologica 103 73-78.
Rao G.S., Breuer H. and Witschi E. (1969). In vitro conversion of 17alpha-hydroxyprogesterone to androstenedione by mashed gonads from metamorphic stages of Xenopus laevis. Gen. Comp. Endocrinol. 12 119-123.
Rawlinson P.A. (1974). Biogeography and ecology of the reptiles of Tasmania and the Bass Strait area. In Biogeography and Ecology in Tasmania. (W.D. Williams, Ed.). pp230-269. W. Junk, The Hague.
Rhoades R. and Pflanzer R. (1992). Human Physiology. (Second edition). Saunders College Publishing, USA.
Riley D. and Callard I.P. (1988). An estrogen receptor in the liver of the viviparous watersnake, Nerodia; characterisation and seasonal changes in binding capacity. Endocrinology 123 753-761.
Rostal D.C., Owens D.Wm., Grumbles J.S., MacKenzie D.S. and Amoss M.A. Jr. (1998). Seasonal reproductive cycle of the Kemp's Ridley sea turtle (Lepidochelys kempi). Gen. Comp. Endocrinol. 109 232-243.
Ruokonen A. (1978). Steroid metabolism in testis tissue: the metabolism of pregnenolone, pregnenolone sulphate, dehydroepiandrosterone and dehydroepiandrosterone sulphate in human and boar testis in vitro. J. Steroid Biochem. 9 939-946.
Ruokonen A. and Vihko R. (1974). Steroid metabolism in testis tissue: concentrations of unconjugated and sulfated neutral steroids in boar testis. J. Steroid Biochem. 5 33-38.
Saint Girons H. (1985). Comparative data on lepidosaurian reproduction and some time tables. In Biology of the Reptilia. Development B. (C. Gans and F. Billet, Eds). Vol 15. pp 35-58. Wiley, New York.
Saint Girons H., Bradshaw S.D. and Bradshaw F.J. (1993). Sexual activity and plasma levels of sex steroids in the aspic viper Vipera aspis L. (Reptilia, Viperidae). Gen. Comp. Endocrinol. 91 287-297.
Sakai N., Ueda H., Suzuki N. and Nagahama Y. (1989). Steroid production by imago salmon (Oncorhynchus rhodurus) testes at different developmental stages. Gen. Comp. Endocrinol. 75 231-240.
Sandor T., Lehoux J.G. and Mehdi A.Z. (1972). Comparative enzymology of steroid-hydroxylating systems of non-mammalian vertebrate adrenal tissue. Gen. Comp. Endocrinol. Suppl. 3 279-288.
Sanyal M.K. and Prasad M.R.N. (1967). Reproductive cycle of the Indian house lizard, Hemidactylus flaviviridis Ruppell. Copeia 1967 627-633.
Satrawaha R. and Bull C.M. (1981). The area occupied by an omnivorous lizard, Trachydosaurus rugosus. Aust. Wildl. Res. 8 435-442.
Schulster D., Burstein S. and Cooke B.A. (1976). Molecular endocrinology of the steroid hormones. John Wiley and Sons, London.
Schlinger B.A. and Callard G.V. (1990). Aromatization mediates aggressive behavior in quail. Gen. Comp. Endocrinol. 79 39-53.
Schlinger B.A., Fivizzani A.J. and Callard G.V. (1989). Aromatase, 5alpha- and 5beta-reductase in brain, pituitary and skin of the sex-reversed Wilson's pharalope. J. Endocrinol. 122 573-581.
Schoonen W.G.E.J. and Lambert J.G.D. (1986a). Steroid metabolism in the seminal vesicles of the African catfish, Clarias gariepinus (Burchell), during the spawning season, under natural conditions, and kept in ponds. Gen. Comp. Endocrinol. 61 355-367.
Schoonen W.G.E.J. and Lambert J.G.D. (1986b). Steroid metabolism in the testes of the African catfish, Clarias gariepinus (Burchell), during spawning season, under natural conditions and kept in ponds. Gen. Comp. Endocrinol. 64 40-52.
Schoonen W.G.E.J. and Lambert J.G.D. (1987). Gas chromatographic-mass spectrometric analysis of steroids and steroid glucuronides in the seminal vesicle fluid of the African catfish, Clarias gariepinus. Gen. Comp. Endocrinol. 68 375-386.
Schuett G.W. and Duvall D. (1996). Head lifting by female copperheads, Agkistrodon contortrix, during courtship: potential mate choice. Anim. Behav. 51 367-373.
Schuett G.W., Harlow H.J., Rose J.D., Van Kirk E.A. and Murdoch W.J. (1997). Annual cycle of plasma testosterone in male copperheads, Agkistrodon contortrix (Serpentes, Viperidae): relationship to timing of spermatogenesis, mating, and agonistic behaviour. Gen. Comp. Endocrinol. 105 417-424.
Schulster D., Burstein S. and Cooke B.A. (1976). Molecular Endocrinology of the Steroid Hormones. John Wiley and Sons, London.
Schultz R. (1986). In vitro metabolism of steroid hormones in the liver and in blood cells of male rainbow trout (Salmo gairdneri Richardson). Gen. Comp. Endocrinol. 64 312-319.
Schuurmans A.L.G., Bolt J., Veldscholte J. and Mulder E. (1990). Stimulatory effects of antiandrogens on LNCaP human prostate tumour cell growth, EGF-receptor level and acid phosphotase secretion. J. Steroid Biochem. Molec. Biol. 37 849-853.
Schwarzkopf L. (1996). Decreased food intake in reproducing lizards: a fecundity- dependent cost of reproduction? Aust. J. Ecol. 21 355-362.
Schwarzkopf L. and Shine R. (1991). Thermal biology of reproduction in viviparous skinks, Eulamprus tympanum: why do gravid females bask more? Oecologia 88 562-569.
Scott A. (1985). Messages in evolution. New Scientist 25 July 30-31.
Scott A.P. and Sorensen P.W. (1994). Time course of release of pheromonally active gonadal steroids and their conjugates in ovulatory goldfish. Gen. Comp. Endocrinol. 96 309-323.
Scott A.P. and Vermierssen E.L.M. (1994). Production of conjugated steroids by teleost gonads and their role as pheromones. In Perspectives in Comparative Endocrinology. pp645-654. National Research Council of Canada, Canada.
Seagraves W.A. (1991). Something old, some things new: the steroid receptor superfamily in Drosophila. Cell. 67 225-228.
Secor S.M. (1987). Courtship and mating behaviour of the speckled kingsnake, Lampropeltis getulus holbrooki. Herpetologica. 43 15-28.
Seigel R.A., Loraine R.K. and Gibbons J.W. (1995). Reproductive cycles and temporal variation in fecundity in the black swamp snake, Seminatrix pygaea. Am. Midl. Nat. 134 171-377.
Sekharappa B.M. and Devaraj Sarkar H.B. (1978). Role of corpora lutea in gestation in the skink Mabuya carinata (Schn.). Indian J. Exp. Biol. 16 1097-1098.
Semlitsch R.D. and Moran G.B. (1984). Ecology of the redbelly snake (Storeria occipitomaculata) using mesic habitats in South Carolina. Am. Midl. Nat. 111 33-40.
Shea G.M. (1989). Diet and reproductive biology of the Rottnest Island bobtail, Tiliqua rugosa konowi (Lacertilia, Scincidae). Herpetological J. 1 366-369.
Shea G.M. (1992).The systematics and reproduction of blue-tongued lizards of the genus Tiliqua (Squamata: Scincidae). Ph.D. Thesis, University of Sydney, NSW, Australia.
Shea G.M. (1993). The male reproductive cycle of the eastern blue-tongued lizard Tiliqua scincoides scincoides (Squamata: Scincidae). In Herpetology in Australia, a diverse discipline. (D. Lunney and D. Ayres, Eds). pp397-403. Royal Zoological Society of New South Wales, Sydney.
Sherwood N.M., Kyle A.L., Kreiberg H., Warby C.M., Magnus T.H., Carolsfeld J. and Price W.S. (1991). Partial characterisation of a spawning pheromone in the herring, Clupea harengus pallasi. Can. J. Zool. 69 91-103.
Shine R. (1980). "Costs" of reproduction. Oecologica 46 92-100.
Shine R. (1983). Reptilian viviparity in cold climates: testing the assumptions of an evolutionary hypothesis. Oecologica 57 397-405.
Shine R. (1985). The reproductive biology of Australian reptiles: a search for general patterns. In Biology of Australasian Frogs and Reptiles. (G. Grigg, R. Shine and H. Ehmann, Eds). pp297-303. Surrey Beaty and Sons Pty, Ltd, Australia.
Shine R. and Guillette L.J. Jr. (1988). The evolution of viviparity in reptiles: a physiological model and its ecological consequences. J. Theor. Biol. 132 43-50.
Shine R. And Harlow P. (1993). Maternal thermoregulation influences offspring viability in a viviparous lizard. Oecologica 96 122-127.
Shine R., Haagner G.V., Branch W.R., Harlow P.S. and Webb J.K. (1996b). Natural history of the African shieldnose snake Aspidelaps scutatus (Serpentes, Elapidae). J. Herpetol. 30 361-366.
Shine R., Harlow P.S., Branch W.R. and Webb J.K. (1996a). Life on the lowest branch: sexual dimorphism, diet, and reproductive biology of an African twig snake, Thelotornis capensis (Serpentes: Colubridae). Copeia 1996 290-299.
Shivanandappa T. and Devaraj Sarkar H.B. (1987). Androgenic regulation of epididymal function in the skink, Mabuya carinata (Schn). J. Exp. Zool. 241 369-376.
Smith S.A. and Shine R. (1997). Intraspecific variation in reproductive mode within the scincid lizard Saiphos equalis. Aust. J. Zool. 45 435-445.
Smith S.J., Palmer B.D. and Selcer K.W. (1995). Androgen receptor and aromatase in the turtle oviduct. Amer. Zool. 6A No. 40.
Snowberger E.A. and Stegeman J.J. (1987). Patterns and regulation of estradiol metabolism by hepatic microsomes from two species of marine teleosts. 66 256-265.
Sorensen P.W. and Stacey N.E. (1991). Sex steroids and F prostaglandins serve dual roles as bloodborne hormones and waterborne pheromones in goldfish. Program and abstracts - second international marine biotechnology conference IMBC - '91. p54.
Stacey N.E. and Sorensen P.W. (1986). 17alpha, 20beta-Dihydroxy-4-pregnen-3-one: a steroidal primer pheromone increasing milt volume in the goldfish, Carassius auratus. Can. J. Zool. 64 2412-2417.
Stacey N.E., Kyle A.L. and Liley N.R. (1986). Fish reproductive pheromones. In Chemical Signals in Vertebrates IV. Ecology, Evolution and Comparative Biology. (D. Duvall, D. Muller-Schwarz and R.M. Silverstein, Eds). pp117-133. Plenum Press, New York.
Stacey N.E., Cardwell. J.R., Liley N.R., Scott A.P. and Sorensen P.W. (1994). Hormones as sex pheromones in fish. In Perspectives in Comparative Endocrinology. pp438-448. National Research Council of Canada, Canada.
Staub N.L. and De Beer M. (1997). The role of androgens in female vertebrates. Gen. Comp. Endocrinol. 108 1-24.
Swain R. (1972). The fauna of southwestern Tasmania. Tasm. Yearbook No. 16 56-64.
Swain R. and Jones S.M. (1994). Annual cycle of plasma testosterone and other reproductive parameters in the Tasmanian skink, Niveoscincus metallicus. Herpetologica 54 502-509.
Tam W.H., Phillips J.G. and Lofts B. (1969). Seasonal changes in the in vitro production of testicular androgens by the cobra (Naja naja Linn.). Gen. Comp. Endocrinol. 13 117-125.
Tam W.H., Phillips J.G. and Lofts B. (1972). Seasonal changes in the secretory activity of the adrenal gland of the cobra (Naja naja, Lin.). Gen. Comp. Endocrinol. 19 218-224.
Taylor J.A. (1985). Reproductive biology of the Australian lizard Ctenotus taeniolatus. Herpetologica. 41 408-418.
Tokarz R.R. (1986). Hormonal regulation of male reproductive behavior in the lizard Anolis sagrei: A test of the aromatization hypothesis. Horm. Behav. 20 364-372.
Toyoda F., Tanaka S., Matsuda K. and Kikuyama S. (1994). Hormonal control of response to and secretion of sex attractants in Japanese newts. Physiol. Behav. 55 569-576.
Trauth S.E., Cooper W.E. J., Vitt L.J. and Perrill S.A. (1987). Cloacal anatomy of the broad-headed skink, Eumeces laticeps, with a description of a female pheromonal gland. Herpetologica. 43 458-466.
Uribe M.C.A., Omana M.E.M., Quintero J.E.G. and Guillette L.J. Jr. (1995). Seasonal variation in ovarian histology of the viviparous lizard Scleroporus torquatus torquatus. J. Morphol. 226 103-119.
Uribe M.C.A., Portales G.L.B. and Guillette L.J. Jr. (1996) Ovarian folliculogenesis in the oviparous Mexican lizard Ctenosaura pectinata. J. Morphol. 230 99-112.
Van Den Hurk R., Schoonen W.G.E.J., Van Zoelen G.A., and Lambe" name="eprints.referencetext" />
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<meta content="This thesis documents the annual profiles of the primary reproductive steroids testosterone (T), 17beta-oestradiol (E2) and progesterone (P4), in the reproductive cycles of male and female blue-tongued lizards, Tiliqua nigrolutea. Data collected from a large captive population over three consecutive reproductive seasons are included. Reproductive cycles are discussed in the context of other viviparous squamate reptiles, while a broader comparative approach is used to consider patterns of steroid biosynthesis and peripheral metabolism.
The annual patterns of circulating concentrations of T, E2 and P4 have been characterised for both sexes. In males, peak plasma T (10.9 +/- 3.00 ng ml-1) and E2 (778.0 +/- 120.00 pg ml-1) concentrations occur coincident with late spermatogenesis and observations of mating, respectively. Plasma P4 concentrations remain basal (< 1.2 ng ml-1) throughout the annual reproductive cycle. In females, increasing plasma E2 concentrations (275.2 +/- 33.87 pg ml-1 - 715.1 +/- 106.68 pg ml-1) are associated with vitellogenesis and plasma T peaks (6.3 +/- 0.63 ng ml-1) in the mating and peri-ovulatory period. In pregnant females, plasma P4 concentrations are elevated for the first two thirds of gestation, peaking in the second trimester at 12.7 +/- 1.27 ng ml-1 and falling rapidly prior to parturition. Concurrently, plasma P4 concentrations in non-reproductively active adult females remain basal (1 - 2 ng ml-1) throughout the year.
There is good circumstantial evidence for a multiennial reproductive cycle in females. Parturition occurs late in the active season, presumably leaving little time for females to store sufficient fat reserves to become vitellogenic in the following spring: reproductive opportunities are effectively missed in at least one year following a reproductive effort. Observed reproductive behaviours, including agonistic male - male interactions, mating, and parturition, are documented.
An investigation of gonadal steroid biosynthetic pathways in this viviparous squamate is presented. This compares variation in the relative contributions of the delta-4 and delta-5 steroidogenic pathways according to sex and reproductive condition. The delta-4 pathway predominates in both sexes, aligning this species phylogenetically with other reptiles. However, there are clear differences between sexes and with changing reproductive condition in the patterns of production of pathway intermediates and end-products. Additionally, detection of a possibly novel polar steroid as a major end-product of steroid biosynthesis in both sexes is reported.
Peripheral (extragonadal) metabolism of T and E2 in a number of reproductively relevant steroid target tissues is compared at times of year chosen to represent three clearly distinctive reproductive conditions in each sex. There are differences both between sexes, between tissue types and with changing reproductive condition in the relative proportions of steroid conjugates and non-conjugated derivatives produced. Biosynthetic pathway activity and peripheral steroid metabolism both appear to be plastic in response to changing reproductive condition in Tiliqua nigrolutea.
With a comprehensive database of information about the reproductive endocrinology and physiology of Tiliqua nigrolutea, this species is now available as a model to further examine selected aspects of the steroid hormone control of reproductive physiology and behaviour in a cool temperate, viviparous reptile.
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<h1 class="ep_tm_pagetitle">Steroids and Reproductive Biology in the Blotched Blue-tongued Lizard, Tiliqua nigrolutea</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Edwards, Ashley</span> (2000) <xhtml:em>Steroids and Reproductive Biology in the Blotched Blue-tongued Lizard, Tiliqua nigrolutea.</xhtml:em> PhD thesis, University of Tasmania.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_679' );" href="http://eprints.utas.edu.au/667/1/front_matter_ashley.pdf" onmouseout="EPJS_HidePreview( event, 'doc_preview_679' );"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a><div class="ep_preview" id="doc_preview_679"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/667/thumbnails/1/preview.png" class="ep_preview_image" border="0" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/667/1/front_matter_ashley.pdf"><span class="ep_document_citation">PDF (01: Front Matter)</span></a> - Requires a PDF viewer<br />62Kb</td></tr><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_680' );" href="http://eprints.utas.edu.au/667/2/whole_thesis.pdf" onmouseout="EPJS_HidePreview( event, 'doc_preview_680' );"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a><div class="ep_preview" id="doc_preview_680"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/667/thumbnails/2/preview.png" class="ep_preview_image" border="0" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/667/2/whole_thesis.pdf"><span class="ep_document_citation">PDF (02: Whole Thesis)</span></a> - Requires a PDF viewer<br />2509Kb</td></tr></table><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">This thesis documents the annual profiles of the primary reproductive steroids testosterone (T), 17beta-oestradiol (E2) and progesterone (P4), in the reproductive cycles of male and female blue-tongued lizards, Tiliqua nigrolutea. Data collected from a large captive population over three consecutive reproductive seasons are included. Reproductive cycles are discussed in the context of other viviparous squamate reptiles, while a broader comparative approach is used to consider patterns of steroid biosynthesis and peripheral metabolism.
The annual patterns of circulating concentrations of T, E2 and P4 have been characterised for both sexes. In males, peak plasma T (10.9 +/- 3.00 ng ml-1) and E2 (778.0 +/- 120.00 pg ml-1) concentrations occur coincident with late spermatogenesis and observations of mating, respectively. Plasma P4 concentrations remain basal (< 1.2 ng ml-1) throughout the annual reproductive cycle. In females, increasing plasma E2 concentrations (275.2 +/- 33.87 pg ml-1 - 715.1 +/- 106.68 pg ml-1) are associated with vitellogenesis and plasma T peaks (6.3 +/- 0.63 ng ml-1) in the mating and peri-ovulatory period. In pregnant females, plasma P4 concentrations are elevated for the first two thirds of gestation, peaking in the second trimester at 12.7 +/- 1.27 ng ml-1 and falling rapidly prior to parturition. Concurrently, plasma P4 concentrations in non-reproductively active adult females remain basal (1 - 2 ng ml-1) throughout the year.
There is good circumstantial evidence for a multiennial reproductive cycle in females. Parturition occurs late in the active season, presumably leaving little time for females to store sufficient fat reserves to become vitellogenic in the following spring: reproductive opportunities are effectively missed in at least one year following a reproductive effort. Observed reproductive behaviours, including agonistic male - male interactions, mating, and parturition, are documented.
An investigation of gonadal steroid biosynthetic pathways in this viviparous squamate is presented. This compares variation in the relative contributions of the delta-4 and delta-5 steroidogenic pathways according to sex and reproductive condition. The delta-4 pathway predominates in both sexes, aligning this species phylogenetically with other reptiles. However, there are clear differences between sexes and with changing reproductive condition in the patterns of production of pathway intermediates and end-products. Additionally, detection of a possibly novel polar steroid as a major end-product of steroid biosynthesis in both sexes is reported.
Peripheral (extragonadal) metabolism of T and E2 in a number of reproductively relevant steroid target tissues is compared at times of year chosen to represent three clearly distinctive reproductive conditions in each sex. There are differences both between sexes, between tissue types and with changing reproductive condition in the relative proportions of steroid conjugates and non-conjugated derivatives produced. Biosynthetic pathway activity and peripheral steroid metabolism both appear to be plastic in response to changing reproductive condition in Tiliqua nigrolutea.
With a comprehensive database of information about the reproductive endocrinology and physiology of Tiliqua nigrolutea, this species is now available as a model to further examine selected aspects of the steroid hormone control of reproductive physiology and behaviour in a cool temperate, viviparous reptile.
</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Thesis (PhD)</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">Majority now published in A1 journals</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270604.html">270000 Biological Sciences > 270600 Physiology > 270604 Comparative Physiology</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270603.html">270000 Biological Sciences > 270600 Physiology > 270603 Animal Physiology - Systems</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270503.html">270000 Biological Sciences > 270500 Zoology > 270503 Animal Anatomy and Histology</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">667</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Ashley Edwards</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">02 Feb 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=667;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=667">item control page</a></p>
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